Immunofixation electrophoresis and electrofocusing.
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چکیده
منابع مشابه
Serum protein electrophoresis and immunofixation by a semiautomated electrophoresis system.
Semiautomated agarose electrophoresis and immunofixation performed with Hydrasys-Hyrys (Sebia) were compared with conventional, manually performed methods, including cellulose acetate electrophoresis, immunoelectrophoresis, and immunofixation. Reference intervals for agarose electrophoresis with Hydrasys-Hyrys were determined. Within-run imprecision (CV) for fraction quantitation with the semia...
متن کاملElectrophoresis and Electrofocusing of Phytochrome from Etiolated Avena sativa L
R udolf Schendel and W olfhart Rüdiger Botanisches Institut der Universität München Z. Naturforsch. 44c, 12—18 (1989); received November 25, 1988 Microheterogeneity, Pest Sequence, Phytochrome Denaturation, Protein Conformation Phytochrome from etiolated oat seedlings (Avena sativa L.) was investigated by “native” gel electrophoresis and by isoelectric focusing. At pH 8 . 8 the Pfr form migrate...
متن کاملImmunofixation. I. General principles and application to agarose gel electrophoresis.
Immunofixation offers the worker an economical means of physically locating a protein in an electrophoretic strip and is ideally suited to forensic medicine, genetic studies, or research. The method is as simple and economical as the commonly used one- or two-dimensional immunoelectrophoresis, yet yields considerably more information.
متن کاملUrine protein electrophoresis and immunofixation electrophoresis supplement one another in characterizing proteinuria.
Urine protein electrophoresis (UPE) is often considered to have limited usefulness in evaluating proteinuria that is not associated with gammopathies. Unusual protein bands that are detected by UPE are commonly characterized by immunofixation electrophoresis (IFE). In this paper, electrophoretic gel patterns are shown to illustrate the greater sensitivity of IFE, compared to UPE. However, UPE r...
متن کاملBence Jones proteins identified by immunofixation electrophoresis of concentrated urine.
addressed some recently recognized problems in testing concentrated urine for monoclonal free light chains of immunoglobulins, i.e., Bence Jones protein (BJP), by high-resolution elec-trophoresis (lIRE) and immunofix-ation electrophoresis (IFE). When BJP is present in low concentrations, poly-clonal free light chains can interfere with identification of the protein either by causing a dense bac...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1982
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/28.8.1797